Link: https://www.ncbi.nlm.nih.gov/pubmed/30856517

J Surg Res. 2019 Mar 8;239:242-252. doi: 10.1016/j.jss.2019.02.015. [Epub ahead of print]
Heterogeneous Pulmonary Response After Tracheal Occlusion: Clues to Fetal Lung Growth.
Dobrinskikh E1, Al-Juboori SI2, Shabeka U2, Reisz JA3, Zheng C3, Marwan AI4.
Author information
Abstract
BACKGROUND:
Understanding inconsistent clinical outcomes in infants with severe congenital diaphragmatic hernia (CDH) after tracheal occlusion (TO) is a crucial step for advancing neonatal care. The objective of this study is to explore the heterogeneous airspace morphometry and the metabolic landscape changes in fetal lungs after TO.

METHODS:
Fetal lungs on days 1 and 4 after TO were examined using mass spectrometry-based metabolomics, fluorescence lifetime imaging microscopy (FLIM), the number of airspaces, and tissue-to-airspace ratio (TAR).

RESULTS:
Two morphometric areas were identified in TO lungs compared with controls (more small airspaces at day 1 and a higher number of enlarged airspaces at day 4). Global metabolomics analysis revealed a significant upregulation of glycolysis and a suppression of the tricarboxylic acid cycle in day 4 TO lungs compared with day 1 TO lungs. In addition, there was a significant increase in polyamines involved in cell growth and proliferation. Locally, FLIM analysis on day 1 TO lungs demonstrated two types of heterogeneous zones-similar to control and with increased oxidative phosphorylation. FLIM on day 4 TO lungs demonstrated appearance of zones with enlarged airspaces and a metabolic shift toward glycolysis, accompanied by a decrease in the FLIM "lipid-surfactant" signal.

CONCLUSIONS:
In normal fetal lungs, we report a novel temporal pattern of varied morphometric and metabolic changes. Initially, there is formation of zones with small airspaces, followed by airspace enlargement over time. Metabolically day 1 TO lungs have zones with increased oxidative phosphorylation, whereas day 4 TO lungs have a shift toward glycolysis in the enlarged airspaces. Based on our observations, we speculate that the "best responders" to tracheal occlusion should have bigger lungs with small airspaces and normal surfactant production.

Copyright © 2019 Elsevier Inc. All rights reserved.

KEYWORDS:
Congenital diaphragmatic hernia; Fluorescence lifetime imaging microscopy; Heterogeneous metabolic zones; Metabolic landscape; Pulmonary hypoplasia; Tracheal occlusion

PMID: 30856517 DOI: 10.1016/j.jss.2019.02.015
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