Link: http://www.ncbi.nlm.nih.gov/pubmed/26720608

Pediatr Res. 2016 May;79(5):766-75. doi: 10.1038/pr.2015.277. Epub 2015 Dec 31.
The transcriptome of nitrofen-induced pulmonary hypoplasia in the rat model of congenital diaphragmatic hernia.
Mahood TH1, Johar DR1,2, Iwasiow BM1,3, Xu W4,5, Keijzer R1,3.
Author information

Abstract
BACKGROUND:
We currently do not know how the herbicide nitrofen induces lung hypoplasia and congenital diaphragmatic hernia in rats. Our aim was to compare the differentially expressed transcriptome of nitrofen-induced hypoplastic lungs to control lungs in embryonic day 13 rat embryos before the development of embryonic diaphragmatic defects.
METHODS:
Using next-generation sequencing technology, we identified the expression profile of microRNA (miRNA) and mRNA genes. Once the dataset was validated by both RT-qPCR and digital-PCR, we conducted gene ontology, miRNA target analysis, and orthologous miRNA sequence matching for the deregulated miRNAs in silico.
RESULTS:
Our study identified 186 known mRNA and 100 miRNAs which were differentially expressed in nitrofen-induced hypoplastic lungs. Sixty-four rat miRNAs homologous to known human miRNAs were identified. A subset of these genes may promote lung hypoplasia in rat and/or human, and we discuss their associations. Potential miRNA pathways relevant to nitrofen-induced lung hypoplasia include PI3K, TGF-β, and cell cycle kinases.
CONCLUSION:
Nitrofen-induced hypoplastic lungs have an abnormal transcriptome that may lead to impaired development.
PMID: 26720608 [PubMed - in process]